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KMID : 0545120180280071068
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Journal of Microbiology and Biotechnology
2018 Volume.28 No. 7 p.1068 ~ p.1077
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Genetic and functional analysis of the DKxanthene biosynthetic gene cluster from Myxococcusstipitatus DSM 14675
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Hyun Hye-Sook
Lee Sun-Jin
Lee Jong-Suk
Cho Kyung-Yun
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Abstract
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DKxanthenes area class of yellow secondary metabolites produced by myxobacterial genera Myxococcusand Stigmatella. We identified a putative 49.5 kb DKxanthene biosynthetic gene cluster from M. stipitatus DSM 14675 by genomic sequence and mutational analysis. The cluster was comprisedof 15 genes (MYSTI_06004-MYSTI_06018) encoding polyketide synthases, non-ribosomal peptide synthases, and proteins with unknown functions. Disruption of the genes by plasmid insertion resulted in defects in the production of yellow pigments. High-performance liquid chromatography and liquid chromatography-tandem mass spectrometryanalysis indicated that the yellow pigments produced by M. stipitatus DSM 14675 might be noble DKxanthene derivatives.M. stipitatus did not require DKxanthenes for the formation of heat-resistant viable spores,unlike M. xanthus. Furthermore, DKxanthenes showed growth inhibitory activity against the fungi Aspergillusniger, Candida albicans, and Rhizopusstolonifer.
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KEYWORD
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Myxobacteria, Myxococcus stipitatus, secondary metabolite, DKxanthene, biosynthetic gene
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